Novel cell culture protocol developed to induce mouse embryonic stem cell differentiation
Researchers have developed a new, more accurate, method to stimulate mouse embryonic stem (ES) cell differentiation with increased expression of LIM homeobox 1 (Lhx1) – a key biomarker.
Researchers from ETH Zürich (Switzerland) have developed a novel cell culture protocol that can stimulate the differentiation of mouse embryonic stem (ES) cells – providing a new avenue for stem cell research and development.
A pre-print of their research was recently published on bioRxiv.
Previously, researchers have induced the differentiation of mouse ES cells into spermatogonial stem cells (SSCs) through chemical intervention. However, these cells lacked an enhanced expression of SSC biomarkers, such as LIM homeobox 1 (Lhx1). This shortfall suggests that some important aspects of SSC development have not yet been replicated accurately in vitro.
The team have now developed a novel cell culture protocol that can, in combination with chemical intervention, stimulate mouse ES cell differentiation into cells with spermatogonia-like morphology (CSMs) in vitro.
In their study, the team used the protein leukemia inhibitory factor to remove GSK3β and MEK and employed a specific schedule of partial medium replacements.
When combining this protocol with the previously reported chemical intervention procedure, the differentiation process showed a different expression of spermatogonia- and gonocyte-specific genes.
For the first time, the team saw an increase inLhx1expression when compared to a control – suggesting that thisin vitroES differentiation method is more accurate than ever seen before.
This research has provided new insights into stem cell development and may advance our ability to study and manipulate aspects of spermatogonia production.
Ref:https://www.regmednet.com/novel-cell-culture-protocol-developed-to-induce-mouse-embryonic-stem-cell-differentiation/
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