Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing
Cryopreservation is routinely used for the long-term storage of cells in various areas of academic, industrial, and clinical research.
Cryopreservation is routinely used for the long-term storage of cells in various areas of academic, industrial, and clinical research. To keep frozen cells alive, it is necessary to vitrify (or nanocrystallize) water on the inside and outside of the cells. Vitrification is conventionally achieved by adding at least one cryoprotective agent (CPA) to the medium. However, CPAs should ideally be avoided due to their cytotoxicity and potential side effects on the cells. Herein, we demonstrate a method of CPA-free cryopreservation, in which cells are almost vitrified by ultrarapid cooling using inkjet printing. The freezing method should be generally suitable for all kinds of cells that are susceptible to CPAs, including stem cells.
Reference:https://www.pnas.org/content/116/16/7738.short
ارسال به دوستان